Immobilization of chemically modified horse radish peroxidase within activated alginate beads
نویسندگان
چکیده
منابع مشابه
Immobilization of antibodies on alginate-chitosan beads.
An anti-hapten IgG was covalently immobilized on glutaraldehyde-activated alginate-chitosan gel beads. The antibody immobilization efficiency was influenced by glutaraldehyde-bead reaction time, IgG concentration and pH. In addition, immobilization conditions such as glutaraldehyde and antibody concentrations influenced antibody hapten binding affinity. The immobilized IgG on the beads was stab...
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The present study describes the immobilization of horseradish peroxidase (HRP) on magnetite-modified polyaniline (PANImG) activated with glutaraldehyde. After the optimization of the methodology, the immobilization of HRP on PANImG produced the same yield (25%) obtained for PANIG with an efficiency of 100% (active protein). The optimum pH for immobilization was displaced by the effect of the pa...
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Background: Although advantages of immobilization of cells through entrapment in calcium alginate gel beads have already been demonstrated, nevertheless, instability of the beads and the mass transfer limitations remain as the major challenges.Objective: The objective of the present study was to increase the stability, porosity (reduce mass transfer limit...
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متن کاملModified alginate matrices for the immobilization of bioactive agents.
Bioactive agents (catalase - an enzyme, and nisin - a bacteriocin) were covalently immobilized on alginate activated with sodium periodate (oxidatively converting 2,3-dihydroxy groups into dialdehyde residues), followed or preceded by ionotropic gelation. For the same protein coupling yield, the retained enzyme activity of the immobilized enzyme (ImE) can be markedly increased by diminishing th...
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ژورنال
عنوان ژورنال: Hemijska industrija
سال: 2014
ISSN: 0367-598X,2217-7426
DOI: 10.2298/hemind121122036s